Identifying Micro-Organisms

AIM: to practice using some sterile techniques, to register a range of micro-organisms found in spread macroscopically and to examine a range of micro- organisms found in intellectual nourishment and water supply macroscopically APPARATUS:         Incubator          impotent nutritive agar plates 1.         3 water ensamples 2.         1 air sample 3.         3 food samples          etna burner         Sterile pipettes          maintain loops         Sticky tape         Marking tape         Water samples(various)          yoghurt         Inoculating needle/ probe          sick from fruit, vegetables or chou         Methylated strong drink RISK ASSESMENT: 1.         Glassw are - dejection cause cuts if handled incorrectly 2.         Bunsen burner - can cause fire if not on caoutchouc set fire to 3.         Methylated pot likker - toxic if ingested, highly combustible. Skin irritant 4.         Although the micro-organisms utilize in the activities in this manual are considered safe, standard microbiological appendage requires that they are regarded as potentially harmful SAFETY PROCEDURES: 1.         Handle with care, do not pull alone glassware near the distinctness of the bench 2.         take care, do not leave flame unattended keep flammable liquid i.e. Methylated enliven away 3.         Keep Methylated spirits away from flames. accept gloves when handling it 4.         Wear gloves, lab coat and safety glasses. alone used equipment should be placed in disinfectant.

Dispose of cultures after use, in an autoclave. Wash hands forrader leaving lab. never open agar plate after setting up METHOD: 1.         Sterilise the workbench area by take in alcohol on to it 2.         collect 8 sterile food agar plates 3.         A sample of fresh-cut milk was obtained 4.         leave one plate aside, certain and taped as the make 5.          use a sterile inoculating loop, a undersized sample of the fresh milk was streaked onto the plates. This plate was then(prenominal) sealed with sticky tape and labeled as fresh milk 6.          travel 3 and 4 were then repeat with tap water, distilled water, seek tank water, fresh milk, old milk, If you requirement to get a full essay, order it on ou r website: BestEssayCheap.com

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